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Literature summary extracted from

  • Luo, L.; Soosaipillai, A.; Grass, L.; Diamandis, E.P.
    Characterization of human kallikreins 6 and 10 in ascites fluid from ovarian cancer patients (2006), Tumour Biol., 27, 227-234.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.21.B10 medicine active hK6 is present in ovarian cancer ascites fluid, provides a rationale for the development of novel therapeutic strategies Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.B10 additional information activity of hK6 can be suppressed by ca. 60% by monoclonal antibody E24 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.B10 0.23
-
 Pro-Phe-Arg-7-amido-4-methylcoumarin purified hK6 Homo sapiens
3.4.21.B10 0.3
-
 Phe-Ser-Arg-7-amido-4-methylcoumarin purified hK6 Homo sapiens
3.4.21.B10 0.36
-
 Val-Pro-Arg-7-amido-4-methylcoumarin purified hK6 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.B10 30000
-
 purified hK6, Western blot analysis Homo sapiens
3.4.21.B41 30000
-
 SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.B10 Homo sapiens
-
-
-
3.4.21.B41 Homo sapiens O43240
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.B10 hK6 purified on immunoaffinity columns, followed by reverse-phase high performance liquid chromatography, to homogeneity Homo sapiens
3.4.21.B41 reverse-phase high-performance liquid chromatography and immunoaffinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.B10 additional information ovarian cancer ascites fluid from ovarian cancer patients during therapeutic drainage. hK6 and hK10 concentrations are positively correlated in ovarian cancer ascites fluid, majority of hK6 present in the free (uncomplexed) form Homo sapiens
-
3.4.21.B41 ascites fluid all K10 present in ovarian cancer ascites fluids are in the uncomplexed, zymogen form and have no detectable enzymatic activity Homo sapiens
-
3.4.21.B41 ovary cancer cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.B10 additional information hK6 shows weak trypsin-like enzymatic activity, does not cleave Val-Leu-Lys-7-amido-4-methylcoumarin, Glu-Lys-Lys-7-amido-4-methylcoumarin and Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin Homo sapiens ?
-
 ?
3.4.21.B10 Phe-Ser-Arg-7-amido-4-methylcoumarin purified hK6 Homo sapiens Phe-Ser-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.B10 Pro-Phe-Arg-7-amido-4-methylcoumarin purified hK6 Homo sapiens Pro-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.B10 Val-Pro-Arg-7-amido-4-methylcoumarin purified hK6 Homo sapiens Val-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.B41 additional information kallikrein 10 from asites fluid does not cleave L-Val-Pro-Arg-7-amido-4-methylcoumarin, L-Phe-Ser-Arg-7-amido-4-methylcoumarin, L-Pro-Phe-Arg-7-amido-4-methylcoumarin, L-Val-Leu-Lys-7-amido-4-methylcoumarin, L-Glu-Lys-Lys-7-amido-4-methylcoumarin, and L-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.B10 ? x * 30000, SDS-PAGE, purified hK6 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.21.B10 hK6
-
 Homo sapiens
3.4.21.B10 kallikrein 6
-
 Homo sapiens
3.4.21.B41 kallikrein 10
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.B10 0.0003
-
 Pro-Phe-Arg-7-amido-4-methylcoumarin purified hK6 Homo sapiens
3.4.21.B10 0.00083
-
 Phe-Ser-Arg-7-amido-4-methylcoumarin purified hK6 Homo sapiens
3.4.21.B10 0.0013
-
 Val-Pro-Arg-7-amido-4-methylcoumarin purified hK6 Homo sapiens